Photometrics Prime BSI Scientific CMOS Camera
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Photometrics Prime BSI Scientific CMOS Camera

The Prime BSI Scientific CMOS camera delivers the perfect balance between high resolution imaging and sensitivity with an optimized pixel design and near perfect 95% Quantum Efficiency. 

This 4 Megapixel camera with 6.5µm pixels captures highly detailed images at high frame rates, making it the most versatile sCMOS for live cell imaging techniques, including Total Internal Reflection Fluorescence (TIRF), Ratiometric Imaging and Lightsheet microscopy.

For more information, please visit: www.photometrics.com

Photometrics Prime BSI Scientific CMOS Camera
High Quantum Efficiency 95% Peak QE

Maximises ability to detect weak signals, enables short exposure times for high frame rates, minimizes phototoxicity across a wide range of wavelengths

Optimized 6.5µm Pixel Size

Maximise light collection while maintaining proper spatial sampling at 60X

Extremely Low Read Noise

Maximise your ability to detect faint fluorescence

Fast Frame Rates

Capture highly dynamic events with high temporal resolution

Large Field of View

Maximise the number of cells that can be tracked and monitored per frame

PrimeEnhance™

Real-time quantitative denoising algorithm that improves image clarity by reducing photon-shot (Poisson) noise | Delivers an increase in Peak Signal to Noise Ratio of 3X

PrimeLocate™

Dynamically evaluates and acquires only the relevant data for localization based super-resolution applications

Enhanced Dynamic Range

Measure both bright and dim signal levels within the same image | 35,000:1 Dynamic Range (91 dB)

Multiple Expose Out Triggering

Control up to four light sources for multi-wavelength acquisitions

SMART Streaming™

Faster acquisition rates with variable exposures, ideal for multi-probed live cell imaging | Compatible with Multiple Expose Out Triggering

Take a look at our Camera Comparison Guide


Prime BSI delivers a 100% pixel fill factor and does not rely on micro-lensing technology to increase detection, resulting in a 30% increase in sensitivity over previous sCMOS cameras.

This perfect balance in performance makes the Prime BSI the most versatile imaging camera for live-cell imaging with:

  • Highest Sensitivity
  • High Resolution
  • Large Field of View
  • High Frame Rates
  • Large Dynamic Range


Schematics

Accessories

Included

  • USB 3.0 Cable
  • Trigger Cable
  • Power Supply
  • Manual
  • QuickStart Guide

Additional

  • PCIe Card/Cable 
  • Liquid Circulator 
  • Liquid Cooling Tubes

Primary Applications

  • TIRF Microscopy
  • Ratiometric Imaging
  • Cell Motility
  • Light Sheet Microscopy

Sensor
Sensor
Gpixel GSENSE2020BSI Scientific CMOS sensor
Active Array Size
Active Array Size
2048 x 2048 (4.2 Megapixel)
Pixel Area
Pixel Area
6.5μm x 6.5μm (42.25μm2
Sensor Area
Sensor Area
13.3mm x 13.3mm | 18.8mm diagonal
Peak QE%
Peak QE%
>95%
Read Noise
Read Noise
1.3e- (Median) | 1.5e- (RMS)
Full-Well Capacity
Full-Well Capacity
45,000e- (Combined Gain), 10,000e- (High Gain)
Dynamic Range
Dynamic Range
35,000:1 (Combined Gain)
Bit Depth
Bit Depth
16-bit (Combined Gain), 11-bit (High Gain)
Readout Mode
Readout Mode
Rolling Shutter | Effective Global Shutter
Binning
Binning
2x2 (on FPGA)
Cooling Performance
Cooling Performance
Sensor Temperature | Dark Current
Air Cooled
Air Cooled
-20ºC @ 30ºC Ambient | 0.5e-/pixel/second
Liquid Cooled
Liquid Cooled
-30ºC @ 30ºC Ambient | 0.12e-/pixel/second
Camera Interface
Camera Interface
Digital Interface
Digital Interface
PCIe, USB 3.0
Lens Interface
Lens Interface
C-Mount
Mounting Points
Mounting Points
2 x ¼ 20” mounting points per side to prevent rotation
Liquid Cooling
Liquid Cooling
Quick Disconnect Ports
Triggering Modes
Triggering Modes
Function
Input Trigger Modes
Input Trigger Modes
Trigger-First: Sequence triggered on first rising edge | Edge: Each frame triggered on rising edge | SMART Streaming: Fast iteration through multiple exposure times
Output Trigger Modes
Output Trigger Modes
Any Row: Expose signal is high while any row is acquiring data | Rolling Shutter: Effective Global Shutter – Expose signal is high when all rows are acquiring data | Signal is High for set Exposure time – Readout Time
Output Trigger Signals
Output Trigger Signals
Expose Out (up to four signals), Read Out, Trigger Ready