The Scientifica FLIM upgrade kit is an integrated and flexible Fluorescence Lifetime Imaging solution. When installed on a Scientifica multiphoton microscope, the FLIM upgrade enables simultaneous fluorescence intensity and fluorescence lifetime imaging, in up to two colour channels, using the versatile Picoquant TCSPC system.
By combining the high temporal resolution of intensity imaging with the detailed information on chemical environment provided by fluorescence lifetime imaging, highly quantitative data can be acquired.
The field of neuroscience has witnessed substantial scientific advances over the last couple of decades, making it feasible for neuroscientists to answer a wide spectrum of questions that were not previously achievable. Here we discuss some techniques that have revolutionised neuroscience.
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