Eyes wide shut – Brain wide up! New research conducted using a Scientifica multiphoton microscope
Multiphoton Microscope

Eyes wide shut – Brain wide up! New research conducted using a Scientifica multiphoton microscope

The Scientifica HyperScope was used by researchers at the University of Bern and Inselspital, to identify how the brain triages emotions during dream sleep to consolidate the storage of positive emotions, while dampening the consolidation of negative ones. The work expands the importance of sleep in mental health and opens new ways of therapeutic strategies.

Research goal

Rapid eye movement (REM or paradoxical) sleep is a unique and mysterious sleep state during which most of the dreams occur together with intense emotional contents. How and why these emotions are reactivated is unclear. The prefrontal cortex integrates many of these emotions during wakefulness but appears paradoxically quiescent during REM sleep. "Our goal was to understand the underlying mechanism and the functions of such a surprising phenomenon", says Prof. Antoine Adamantidis from the Department of Biomedical Research (DBMR) at the University of Bern and the Department of Neurology at the Inselspital, University Hospital of Bern.

Processing emotions, particularly distinguishing between danger and safety, is critical for the survival of animals. In humans, excessively negative emotions, such as fear reactions and states of anxiety, lead to pathological states like Post-Traumatic Stress Disorders (PTSD). In Europe, roughly 15% of the population is affected by persistent anxiety and severe mental illness. The research group headed by Antoine Adamantidis is now providing insights into how the brain helps to reinforce positive emotions and weaken strongly negative or traumatic emotions during REM sleep. This study was published in the journal Science.

A Dual mechanism

The researchers first conditioned mice to recognize auditory stimuli associated with safety and others associated with danger (aversive stimuli). The activity of neurons in the brain of mice was then recorded during sleep-wake cycles. In this way, the researchers were able to map different areas of a cell and determine how emotional memories are transformed during REM sleep.

Neurons are composed of a cell body (soma) that integrates information coming from the dendrites (inputs) and send signals to other neurons via their axons (outputs). The results obtained showed that cell somas are kept silent while their dendrites are activated. "This means a decoupling of the two cellular compartments, in other words soma wide asleep and dendrites wide awake", explains Adamantidis. This decoupling is important because the strong activity of the dendrites allows the encoding of both danger and safety emotions, while the inhibitions of the soma completely blocks the output of the circuit during REM sleep. In other words, the brain favours the discrimination of safety versus danger in the dendrites, but block the over-reaction to emotion, in particular danger.

Excitatory neurons in a sleeping mouse - Credits: Dr. M. Aime, Adamantidis Lab 2022. Acquired using the Scientifica HyperScope
Excitatory neurons in a sleeping mouse - Credits: Dr. M. Aime, Adamantidis Lab 2022. Acquired using the Scientifica HyperScope

A survival advantage

According to the researchers, the coexistence of both mechanisms is beneficial to the stability and survival of the organisms: "This bi-directional mechanism is essential to optimise the discrimination between dangerous and safe signals", says Adamantidis. If this discrimination is missing in humans and excessive fear reactions are generated, this can lead to anxiety disorders. The findings are particularly relevant to pathological conditions such as post-traumatic stress disorders, in which trauma is over-consolidated in the prefrontal cortex, day after day during sleep.

Breakthrough for sleep medicine

These findings pave the way to a better understanding of the processing of emotions during sleep in humans and open new perspectives for therapeutic targets to treat maladaptive processing of traumatic memories, such as Post Traumatic Stress Disorders (PTSD) and their early sleepdependent consolidation. Additional acute or chronic mental health issues that may implicate this somatodendritic decoupling during sleep include acute and chronic stress, anxiety, depression, panic, or even anhedonia, the inability to feel pleasure. Sleep research and sleep medicine have long been a research focus of the University of Bern and the Inselspital, Bern University Hospital. "We hope that our findings will not only be of interest to the patients, but also to the broad public", says Adamantidis.

Read the full study

To read the full publication, please click here.

A word from Dr Mattia Aime

"Working with the Scientifica HyperScope was a great experience, allowing us to perform complex experiments such as dual colour imaging and optogenetics stimulations during imaging sessions".

Scientifica HyperScope

Push the boundaries of light microscopy and find the answers to the most intricate biological questions with the HyperScope, a proven multiphoton microscope.

Contact Form

Contact us

*为必须填写内容

选择感兴趣领域