CoolLED pE-800
CoolLED pE-800
CoolLED pE-800

CoolLED pE-800

With eight individually controllable LEDs and fast TTL switching, the pE-800 takes centre stage as the widefield illumination method of choice.

Versatile and intuitive the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.

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产品优势

Versatile

8 selectable channels offers versatility, with any channels selectable simultaneously at 100%

Individual control

Individual control of selected LED wavelengths reduces background.

Broad spectrum of wavelengths

From 365-740 nm, covering DAPI-Cy7.

High-speed imaging

Industry-leading <7 µs TTL triggering for high-speed imaging. Removable inline excitation filter holders: no moving parts for high-speed imaging.

Control

Software, TTL, analogue control options for optimised illumination. Optimised irradiance control in 1 % steps (2-100 %) and fine control from 0-2 %.

Light delivery

Via liquid Light Guide for maximum compatibility.

pE-800 Spectrum Graph

Mix & match with eight channels

Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.

All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores. Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.

Capture high-speed events

Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging. Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8 (USB controlled TTL Conversion Kit).

The ability to fit inline excitation filters capitalises on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.

Beyond imaging

Eight individual analogue inputs allow irradiance to be controlled from 0-100 % for each channel via analogue signal (0-10 v), which is ideal for electrophysiology and optogenetics applications.

Affordable automation with Sequence Runner

The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. Once an LED and irradiance sequence are specified in software, Sequence Runner provides lightning fast synchronised triggering via the global TTL-in of the pE-800 and a single TTL-out from a camera (or other hardware).

The pE-800 is unique in offering the only 8-channel Sequence Runner available, and when combined with inline filters, transforms a manual microscope into an affordable and powerful eight-channel automated imaging system. As laboratory budgets become stretched, the pE-800 presents the ideal cost-effective Illumination System which makes high-end LED technology more accessible to life science researchers.

Environment & Safety

Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:

  • Mercury-free and laser-free
  • Energy Efficient
  • Long lifetime
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

Intuitive software control

Software control is achieved through a fast USB 2.0 connection and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:

  • On/off control
  • LED selection
  • Real time irradiance control
  • Sequence Runner
  • Save and load pre-sets
  • pE-800 start-up settings
  • Analogue settings

Download

Download the CoolLED pE-800 brochure.

Speak to one of our experts for details on pricing, features, installation and support.

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