Scientifica Multiphoton Detection Unit XL (MDU XL)
Scientifica's MDU XL is a multiphoton detection unit with increased sensitivity, designed to achieve a superior signal-to-noise ratio and improved image quality, translating to crisper and deeper images.
The MDU XL offers up to 35% more efficient light collection in multiphoton imaging experiments for up to two colours when compared to the standard MDU.
Features
Optimised for use with a range of large back aperture objectives, meaning the MDU XL enables collection of more scattered light for deeper tissue structures compared to the standard MDU.
The MDU XL can be fitted with two photomultiplier tubes (PMTs) for photon collection from two channels simultaneously.
Available with up to two gated or protected GaAsP PMTs for maximum sensitivity.
Features
Optimised for use with a range of large back aperture objectives, meaning the MDU XL enables collection of more scattered light for deeper tissue structures compared to the standard MDU.
The MDU XL can be fitted with two photomultiplier tubes (PMTs) for photon collection from two channels simultaneously.
Available with up to two gated or protected GaAsP PMTs for maximum sensitivity.
Customer acquired data
Layer V pyramidal neurons in the visual cortex expressing GCaMP6s, in vivo. The laser power was kept at a constant power level while increasing the imaging depth.
NOTE: fluctuations in intensity within a structure are caused by calcium transients during the image stack acquisition.
Three-photon capabilities
The MDU XL offers the ability to carry out three-photon imaging.
Below: mouse olfactory bulb, with neurons expressing GCaMP6. The blood vessels are labelled with Sulforhodamine. Despite the highly scattering properties of the olfactory bulb, with three-photon imaging we are able to clearly image through the dura down to the mitral cells with just 30 mW excitation power at 1320 nm.
Customer acquired data
Layer V pyramidal neurons in the visual cortex expressing GCaMP6s, in vivo. The laser power was kept at a constant power level while increasing the imaging depth.
NOTE: fluctuations in intensity within a structure are caused by calcium transients during the image stack acquisition.
Three-photon capabilities
The MDU XL offers the ability to carry out three-photon imaging.
Below: mouse olfactory bulb, with neurons expressing GCaMP6. The blood vessels are labelled with Sulforhodamine. Despite the highly scattering properties of the olfactory bulb, with three-photon imaging we are able to clearly image through the dura down to the mitral cells with just 30 mW excitation power at 1320 nm.
Design & Specifications
| Collection Optics | Primary Detection Dichroic: 60 x 40 x 1 |
|---|---|
| Collection lens diameter: 45 mm | |
| Maximum collection angle: ~±8° assuming 20mm objective back aperture | |
| IR Blocking Filter: 680nm Shortpass 50mm Ø | |
| Emission Filters | Emission filter: 32mm Ø - one for each detector |
| Short wavelength perpendicular to the incident light, longer wavelength on the incident direction | |
| Blocking filter: 32mm Ø for stimulation wavelength | |
| Photomultiplier Tubes | Two locally mounted detectors of the same type: GaAsP protected photomultiplier tube (H10770PA-40) or GaAsP photomultiplier tube with gating function (H11706P-40) |
| Signal output Connector: SMA | |
| Gate input connector (gated GaAsP only): BNC | |
| Protection Circuitry | Protected GaASP: Integrated protection circuitry to trip PMT when overload detected |
| Gated GaAsP: Voltage gating functionality to avoid overload situations | |
| All systems: Ambient light detector | |
| Objective and Nosepiece Compatibility | Objectives: M32, M27, M25, RMS threaded objectives (<20mm back aperture) |
| Nosepieces: Ships with single objective changer with 2 locking sliders | |
| Compatible with: WI-SRE3 adapter (RMS only) or standard/DIC objective sliders | |
| Preamplifier Bandwidth | Options include: 20 MHz (resonant system), 1 MHz (galvo), or variable bandwidth all with low noise linear power supply |
| Output Connection | BNC |
| PMT Voltage Control | Manual or software control |
| PMT Protection | Integrated protection circuitry in H10771P-40 only. For all PMT options, a remote ambient light sensor is used |
Schematics
Schematics