Scientifica Multiphoton Detection Unit (MDU)
Maximise your photon collection when performing multiphoton fluorescence imaging.
The MDU is an optimised one or two-channel fluorescence collection and detection system specifically designed for multiphoton imaging with small back aperture objectives.
High sensitivity is achieved, as the MDU is available with up to two gated or protected GaAsP photon multiplier tubes (PMTs) and is optimised for integration with Scientifica’s SliceScope microscope.
The MDU has everything you need, comprising of pre-aligned optics, safety shutters/switches, a filter cube and either one or two PMTs. Each detection unit also contains a power supply, preamplifier and control unit.
Increased photon capture
Maximised light collection from a wide range of objectives and condensers. Photons can be harvested from objectives that have either a small or large back apertures (RMS, M25, M27 and M32 thread adapters available).
Above or below the sample
Collect reflected fluorescence through the microscope objective, or transmitted fluorescence using a high-NA oil-immersion condenser lens. Alternatively, combine two MDUs simultaneously for optimum signal collection in up to four channels.
Achieve greater control
With gated GaAsP PMTs, you can have greater control when performing optogenetics experiments. This is because they enable the PMT to be desensitised for pulses of up to 10ms, whilst light stimulation is being applied. This not only optimises your experiments, but increases the lifetime of the PMT, by ensuring unreasonable levels of light are not damaging the amplification stages of the PMT.
Design & Specifications
| Number of channels | Two per MDU |
|---|---|
| Detector types | Bi- and multialkali PMTs, protected and gated GaAsP PMTs |
| Primary dichroic mirror | 665 nm long-pass dichroic |
| Laser blocking filter | 680 nm short-pass filter |
| Maximum objective exit aperture | 24 mm diameter |
| Angular collection | +/- 5 degrees maximum from 20 mm objective, +/- 3.5 degrees from 24 mm objective |
| Objective compatibility | Water-dipping M32X0.75, M27X0.75, M25X0.75 and RMS threaded types (direct or via adapters) |
| Spectral filtration | One Olympus U-MF2 filter cube supplied (un-populated) compatible with standard fluorescence filter sets (25 mm filters and 24 x 36 mm dichroic mirrors, 1 mm thick) |
| Preamplifier bandwidth | 1.35 MHz (galvo detection), 20 MHz (resonant detection) |
| Preamplifier gain | 200,000 (galvo detection), 100,000 (resonant detection) |
| Output connection | SMA (3.5 m signal leads supplied) |
| Inputs | BNC input for gate control on Gated GaAsP variant |
Schematics
Schematics
调查报告
ACS Nano
ACS Nano
The Journal of Physiology
The Journal of Physiology
Cell Reports
Cell Reports