Scientifica Multiphoton Detection Unit (MDU)

An optimised two-channel fluorescence collection and detection system designed for multiphoton fluorescence imaging.

Designed for integration with Scientifica’s SliceScope microscope, the MDU comprises pre-aligned optics, safety shutters/switches, a filter cube and either one or two photomultiplier tubes (PMTs). Each detection unit also contains a power supply, preamplifier and control unit.

Benefits

Customised optics

A custom designed, extra-large fixed position collection lens is sited very close to the objective back aperture, allowing photon collection at the widest of scan angles

High signal to noise ratio

The electronic design of the MDU performs exceptionally by minimising background noise which can degrade the image quality.

Fast filter exchange

A standard Olympus filter cube is fitted to a dovetail slider which can easily move in and out of the MDU, easing the setup of the next set of experiments.

Increased photon capture

Maximised light collection from a wide range of objectives and condensers. Photons can be harvested from objectives that have either a small or large back apertures (RMS, M25, M27 and M32 thread adapters available).

Above or below the sample

Collect reflected fluorescence through the microscope objective, or transmitted fluorescence using a high-NA oil-immersion condenser lens. Alternatively combine two MDUs simultaneously for optimum signal collection in up to four channels.

Design & Specifications

Laser/visible dichroic mirror	Laser/visible dichroic mirror 685 nm long-pass dichroic
Laser blocking filter	Laser blocking filter 680 nm short-pass filter
Maximum objective exit aperture	Maximum objective exit aperture 24 mm diameter
Angular collection	Angular collection +/- 5 degrees maximum from 20 mm objective, +/- 3.5 degrees from 24 mm objective
Objective compatibility	Objective compatibility Water-dipping M32X0.75, M27X0.75, M25X0.75 and RMS threaded types (direct or via adapters)
Spectral filtration	Spectral filtration One Olympus U-MF2 filter cube supplied (un-populated) compatible with standard fluorescence filter sets (25 mm filters and 24 x 36 mm dichroic mirrors, 1 mm thick)
Number of channels	Number of channels Two per MDU
Detector types	Detector types Hamamatsu R9880U-210, R9880U-01, R9880U-20
Preamplifier bandwidth	Preamplifier bandwidth 1.35 MHz (galvo detection), 20 MHz (resonant detection)
Preamplifier gain	Preamplifier gain 200,000 (galvo detection), 100,000 (resonant detection)
Output connection	Output connection SMA (3.5 m signal leads supplied)