Selective and accurate targeting
The industry-leading resolution of the Holostim-3D allows cell bodies and subcellular structures to be specifically targeted with minimal off-target activation. This gives you cleaner, more accurate data across multiple layers of cells.
Analyse larger neuronal networks
Target a greater number of cells in larger brain regions to better understand the function of neuronal networks. The large field-of-view of the HoloStim-3D also allows you to use a higher magnification and resolution objective and still achieve the required field of view.
Freely combine different imaging and stimulation wavelengths
Being able to excite or image target cells expressing different opsins or fluorescent proteins increases your experimental flexibility.
Deeper imaging and stimulation
Specifically target cells and subcellular structures in layer 4 and beyond, achieving high-quality images.
Mimic neuronal firing
You can quickly switch stimulation patterns to better mimic neuronal firing, recreating the natural dynamics within a neural network.
High laser transmission
Minimise sample bleaching and optical damage by activating the same number of cells with a lower power laser.
The modular design means the HoloStim-3D is seamlessly integrated with the Scientifica HyperScope, a proven multiphoton imaging system, so you can easily upgrade to SLM capabilities and start generating high-quality data faster.
Mouse layer ii/iii neurons expressing GCaMP6s (calcium indicator) and C1V1 (red-shifted channelrhodopsin) are simultaneously stimulated using the HoloStim-3D, while their calcium responses are imaged using resonance two-photon imaging on the HyperScope. In the total 100 cells were stimulated at 6 nJ per cell, using a spiral pattern. Data acquired by Kelly Sakaki (Scientifica), Robert Lees and Adam Packer (both University of Oxford).
Investigate neuronal circuits with 3D precision
Discover the roles of neuronal subtypes and brain circuits by simultaneously targeting multiple cells with cellular resolution.
Observe and record comprehensive network activity by combining precise cell stimulation and imaging with the HoloStim-3D.
By selectively photostimulating only the target cells and rapidly switching between stimulation patterns, you can better understand neuronal interactions in networks.
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