Cairn OptoSplit II
The Cairn OptoSplit II emission image splitter is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths, polarisation states or other differentiated states.
Traditionally, dual channel imaging is performed using an electronic filter changer or an additional camera and beamsplitter, neither of which is ideal for all applications. The switching speed of an electronic filter changer limits the temporal resolution, whereas a second camera adds cost and complexity. The OptoSplit uses a unique rotating mirror cradle, which gives adjustable spatial separation, to ensure excellent image registration and features a fully adjustable rectangular aperture to enable cropped sensor imaging modes and reduced scatter.
The latest version uses our own lens design to support the Larger Sensors of scientific CMOS and some EMCCD cameras. The instruments have a correspondingly larger aperture and improved off-axis correction to give enhanced performance with all sensors.
Compact design
With integral C-mount input and output ports
Simple & precise controls
For image registration
Applications
• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, voltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Total Internal Reflection Fluorescence (TIRF)
• Spinning disk confocal
• Single Plane Illumination Microscopy (SPIM)
• Polarisation studies (anisotropy)
• 3D super resolution PALM/STORM (using cylindrical lenses)
• Simultaneous phase contrast / DIC and fluorescence
| Magnifications available | Angled and flat auxiliary drop ins for neutral density filtering, polarisers or chromatic correction• Supports 25mm filters• Supports 2mm thick Ultraflat dichroics along with our recommended size (26x38x2mm)• • |
|---|---|
| Sensor support | For sensors up to 18.8mm diagonal (13.3mm x 13.3mm sensors) |
| Filter support | Supports 25mm filters |
| Dichroic support | Supports 2mm thick Ultraflat dichroics along with Cairn's recommended size (26x38x2mm) |
| Optics | 40mm diameter proprietary optics |
| Coatings | 425nm to 875nm coatings on all surfaces |